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rabbit antihuman bag1  (Proteintech)


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    Proteintech rabbit antihuman bag1
    The OA-associated changes between patients with OA and non-OA controls. (A) The Manhattan-like plot demonstrates the population-specific DE genes between patients with OA and non-OA controls. The numbers on the top panel denote the number of DE genes for each population. The dot size represents the value of −log10 (adjusted p). (B) The subnetworks identified by PhenomeExpress. The DE genes of preHTC were enriched in a pMAPK38 cascade subnetwork. (C) The visualisation of subpopulations of preHTC using UMAP projections, that is, preHTC-1 (blue), preHTC-2 (green), preHTC-3 (brown) and preHTC-4 (red), where a total of 25 778 chondrocytes were analysed by the integrative analysis of all 19 samples. (D) The bar plot shows the significant GO terms for each subpopulation. (E) The Manhattan-like plot shows the zone-specific DE genes between patients with OA and non-OA controls, that is, AS (red), SZ (yellow), MZ (blue) and DZ (green). The numbers on the top panel or bottom panel represent the number of significant upregulated or downregulated DE genes for each zone. (F) The bar plot shows the significant GO terms that were enriched by the zone-specific DE genes. Notably, the MZ-specific and DZ-specific DE genes were not enriched in any biologically meaningful pathways. (G) The subnetworks identified by PhenomeExpress. The DE genes of AS were enriched in response to the chemokine subnetwork and those of SZ were enriched in the regulation of the transcription subnetwork. (H) The top-ranked DE gene of AS or SZ between patients with OA and non-OA controls, <t>BAG1,</t> was validated by IHC staining. (I) The top-ranked DE gene of AS or SZ between patients with OA and non-OA controls, CD9, was validated by IHC staining. The quantification of positive cells from different zones (ie, SZ, MZ and DZ) is displayed by bar plots (replicates n=6), where the AS and SZ were hardly distinguished during IHC staining, therefore, the AS and SZ in Geo-seq results were merged together to only present as SZ in IHC staining. The scale bar: left, 500 μm; right, 50 μm. AS, articular surface; DZ, deep zone; GO, Gene Ontology; IHC, immunohistochemistry; InfC, inflammatory chondrocyte; MZ, middle zone; NS, no significant; OA, osteoarthritis; preInfC, pre-inflammatory chondrocyte; preFC, prefibrocartilage chondrocyte; preHTC, prehypertrophic chondrocyte; SZ, superficial zone; UMAP, uniform manifold approximation and projection. *p<0.05, **p<0.01, ***p<0.001.
    Rabbit Antihuman Bag1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+antihuman+bag1/pmc11187367-376-51-56?v=Proteintech
    Average 93 stars, based on 4 article reviews
    rabbit antihuman bag1 - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Unveiling inflammatory and prehypertrophic cell populations as key contributors to knee cartilage degeneration in osteoarthritis using multi-omics data integration"

    Article Title: Unveiling inflammatory and prehypertrophic cell populations as key contributors to knee cartilage degeneration in osteoarthritis using multi-omics data integration

    Journal: Annals of the Rheumatic Diseases

    doi: 10.1136/ard-2023-224420

    The OA-associated changes between patients with OA and non-OA controls. (A) The Manhattan-like plot demonstrates the population-specific DE genes between patients with OA and non-OA controls. The numbers on the top panel denote the number of DE genes for each population. The dot size represents the value of −log10 (adjusted p). (B) The subnetworks identified by PhenomeExpress. The DE genes of preHTC were enriched in a pMAPK38 cascade subnetwork. (C) The visualisation of subpopulations of preHTC using UMAP projections, that is, preHTC-1 (blue), preHTC-2 (green), preHTC-3 (brown) and preHTC-4 (red), where a total of 25 778 chondrocytes were analysed by the integrative analysis of all 19 samples. (D) The bar plot shows the significant GO terms for each subpopulation. (E) The Manhattan-like plot shows the zone-specific DE genes between patients with OA and non-OA controls, that is, AS (red), SZ (yellow), MZ (blue) and DZ (green). The numbers on the top panel or bottom panel represent the number of significant upregulated or downregulated DE genes for each zone. (F) The bar plot shows the significant GO terms that were enriched by the zone-specific DE genes. Notably, the MZ-specific and DZ-specific DE genes were not enriched in any biologically meaningful pathways. (G) The subnetworks identified by PhenomeExpress. The DE genes of AS were enriched in response to the chemokine subnetwork and those of SZ were enriched in the regulation of the transcription subnetwork. (H) The top-ranked DE gene of AS or SZ between patients with OA and non-OA controls, BAG1, was validated by IHC staining. (I) The top-ranked DE gene of AS or SZ between patients with OA and non-OA controls, CD9, was validated by IHC staining. The quantification of positive cells from different zones (ie, SZ, MZ and DZ) is displayed by bar plots (replicates n=6), where the AS and SZ were hardly distinguished during IHC staining, therefore, the AS and SZ in Geo-seq results were merged together to only present as SZ in IHC staining. The scale bar: left, 500 μm; right, 50 μm. AS, articular surface; DZ, deep zone; GO, Gene Ontology; IHC, immunohistochemistry; InfC, inflammatory chondrocyte; MZ, middle zone; NS, no significant; OA, osteoarthritis; preInfC, pre-inflammatory chondrocyte; preFC, prefibrocartilage chondrocyte; preHTC, prehypertrophic chondrocyte; SZ, superficial zone; UMAP, uniform manifold approximation and projection. *p<0.05, **p<0.01, ***p<0.001.
    Figure Legend Snippet: The OA-associated changes between patients with OA and non-OA controls. (A) The Manhattan-like plot demonstrates the population-specific DE genes between patients with OA and non-OA controls. The numbers on the top panel denote the number of DE genes for each population. The dot size represents the value of −log10 (adjusted p). (B) The subnetworks identified by PhenomeExpress. The DE genes of preHTC were enriched in a pMAPK38 cascade subnetwork. (C) The visualisation of subpopulations of preHTC using UMAP projections, that is, preHTC-1 (blue), preHTC-2 (green), preHTC-3 (brown) and preHTC-4 (red), where a total of 25 778 chondrocytes were analysed by the integrative analysis of all 19 samples. (D) The bar plot shows the significant GO terms for each subpopulation. (E) The Manhattan-like plot shows the zone-specific DE genes between patients with OA and non-OA controls, that is, AS (red), SZ (yellow), MZ (blue) and DZ (green). The numbers on the top panel or bottom panel represent the number of significant upregulated or downregulated DE genes for each zone. (F) The bar plot shows the significant GO terms that were enriched by the zone-specific DE genes. Notably, the MZ-specific and DZ-specific DE genes were not enriched in any biologically meaningful pathways. (G) The subnetworks identified by PhenomeExpress. The DE genes of AS were enriched in response to the chemokine subnetwork and those of SZ were enriched in the regulation of the transcription subnetwork. (H) The top-ranked DE gene of AS or SZ between patients with OA and non-OA controls, BAG1, was validated by IHC staining. (I) The top-ranked DE gene of AS or SZ between patients with OA and non-OA controls, CD9, was validated by IHC staining. The quantification of positive cells from different zones (ie, SZ, MZ and DZ) is displayed by bar plots (replicates n=6), where the AS and SZ were hardly distinguished during IHC staining, therefore, the AS and SZ in Geo-seq results were merged together to only present as SZ in IHC staining. The scale bar: left, 500 μm; right, 50 μm. AS, articular surface; DZ, deep zone; GO, Gene Ontology; IHC, immunohistochemistry; InfC, inflammatory chondrocyte; MZ, middle zone; NS, no significant; OA, osteoarthritis; preInfC, pre-inflammatory chondrocyte; preFC, prefibrocartilage chondrocyte; preHTC, prehypertrophic chondrocyte; SZ, superficial zone; UMAP, uniform manifold approximation and projection. *p<0.05, **p<0.01, ***p<0.001.

    Techniques Used: Immunohistochemistry



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    rabbit antihuman bag1  (Proteintech)
    93
    Proteintech rabbit antihuman bag1
    The OA-associated changes between patients with OA and non-OA controls. (A) The Manhattan-like plot demonstrates the population-specific DE genes between patients with OA and non-OA controls. The numbers on the top panel denote the number of DE genes for each population. The dot size represents the value of −log10 (adjusted p). (B) The subnetworks identified by PhenomeExpress. The DE genes of preHTC were enriched in a pMAPK38 cascade subnetwork. (C) The visualisation of subpopulations of preHTC using UMAP projections, that is, preHTC-1 (blue), preHTC-2 (green), preHTC-3 (brown) and preHTC-4 (red), where a total of 25 778 chondrocytes were analysed by the integrative analysis of all 19 samples. (D) The bar plot shows the significant GO terms for each subpopulation. (E) The Manhattan-like plot shows the zone-specific DE genes between patients with OA and non-OA controls, that is, AS (red), SZ (yellow), MZ (blue) and DZ (green). The numbers on the top panel or bottom panel represent the number of significant upregulated or downregulated DE genes for each zone. (F) The bar plot shows the significant GO terms that were enriched by the zone-specific DE genes. Notably, the MZ-specific and DZ-specific DE genes were not enriched in any biologically meaningful pathways. (G) The subnetworks identified by PhenomeExpress. The DE genes of AS were enriched in response to the chemokine subnetwork and those of SZ were enriched in the regulation of the transcription subnetwork. (H) The top-ranked DE gene of AS or SZ between patients with OA and non-OA controls, <t>BAG1,</t> was validated by IHC staining. (I) The top-ranked DE gene of AS or SZ between patients with OA and non-OA controls, CD9, was validated by IHC staining. The quantification of positive cells from different zones (ie, SZ, MZ and DZ) is displayed by bar plots (replicates n=6), where the AS and SZ were hardly distinguished during IHC staining, therefore, the AS and SZ in Geo-seq results were merged together to only present as SZ in IHC staining. The scale bar: left, 500 μm; right, 50 μm. AS, articular surface; DZ, deep zone; GO, Gene Ontology; IHC, immunohistochemistry; InfC, inflammatory chondrocyte; MZ, middle zone; NS, no significant; OA, osteoarthritis; preInfC, pre-inflammatory chondrocyte; preFC, prefibrocartilage chondrocyte; preHTC, prehypertrophic chondrocyte; SZ, superficial zone; UMAP, uniform manifold approximation and projection. *p<0.05, **p<0.01, ***p<0.001.
    Rabbit Antihuman Bag1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+antihuman+bag1/pmc11187367-376-51-56?v=Proteintech
    Average 93 stars, based on 1 article reviews
    rabbit antihuman bag1 - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    The OA-associated changes between patients with OA and non-OA controls. (A) The Manhattan-like plot demonstrates the population-specific DE genes between patients with OA and non-OA controls. The numbers on the top panel denote the number of DE genes for each population. The dot size represents the value of −log10 (adjusted p). (B) The subnetworks identified by PhenomeExpress. The DE genes of preHTC were enriched in a pMAPK38 cascade subnetwork. (C) The visualisation of subpopulations of preHTC using UMAP projections, that is, preHTC-1 (blue), preHTC-2 (green), preHTC-3 (brown) and preHTC-4 (red), where a total of 25 778 chondrocytes were analysed by the integrative analysis of all 19 samples. (D) The bar plot shows the significant GO terms for each subpopulation. (E) The Manhattan-like plot shows the zone-specific DE genes between patients with OA and non-OA controls, that is, AS (red), SZ (yellow), MZ (blue) and DZ (green). The numbers on the top panel or bottom panel represent the number of significant upregulated or downregulated DE genes for each zone. (F) The bar plot shows the significant GO terms that were enriched by the zone-specific DE genes. Notably, the MZ-specific and DZ-specific DE genes were not enriched in any biologically meaningful pathways. (G) The subnetworks identified by PhenomeExpress. The DE genes of AS were enriched in response to the chemokine subnetwork and those of SZ were enriched in the regulation of the transcription subnetwork. (H) The top-ranked DE gene of AS or SZ between patients with OA and non-OA controls, BAG1, was validated by IHC staining. (I) The top-ranked DE gene of AS or SZ between patients with OA and non-OA controls, CD9, was validated by IHC staining. The quantification of positive cells from different zones (ie, SZ, MZ and DZ) is displayed by bar plots (replicates n=6), where the AS and SZ were hardly distinguished during IHC staining, therefore, the AS and SZ in Geo-seq results were merged together to only present as SZ in IHC staining. The scale bar: left, 500 μm; right, 50 μm. AS, articular surface; DZ, deep zone; GO, Gene Ontology; IHC, immunohistochemistry; InfC, inflammatory chondrocyte; MZ, middle zone; NS, no significant; OA, osteoarthritis; preInfC, pre-inflammatory chondrocyte; preFC, prefibrocartilage chondrocyte; preHTC, prehypertrophic chondrocyte; SZ, superficial zone; UMAP, uniform manifold approximation and projection. *p<0.05, **p<0.01, ***p<0.001.

    Journal: Annals of the Rheumatic Diseases

    Article Title: Unveiling inflammatory and prehypertrophic cell populations as key contributors to knee cartilage degeneration in osteoarthritis using multi-omics data integration

    doi: 10.1136/ard-2023-224420

    Figure Lengend Snippet: The OA-associated changes between patients with OA and non-OA controls. (A) The Manhattan-like plot demonstrates the population-specific DE genes between patients with OA and non-OA controls. The numbers on the top panel denote the number of DE genes for each population. The dot size represents the value of −log10 (adjusted p). (B) The subnetworks identified by PhenomeExpress. The DE genes of preHTC were enriched in a pMAPK38 cascade subnetwork. (C) The visualisation of subpopulations of preHTC using UMAP projections, that is, preHTC-1 (blue), preHTC-2 (green), preHTC-3 (brown) and preHTC-4 (red), where a total of 25 778 chondrocytes were analysed by the integrative analysis of all 19 samples. (D) The bar plot shows the significant GO terms for each subpopulation. (E) The Manhattan-like plot shows the zone-specific DE genes between patients with OA and non-OA controls, that is, AS (red), SZ (yellow), MZ (blue) and DZ (green). The numbers on the top panel or bottom panel represent the number of significant upregulated or downregulated DE genes for each zone. (F) The bar plot shows the significant GO terms that were enriched by the zone-specific DE genes. Notably, the MZ-specific and DZ-specific DE genes were not enriched in any biologically meaningful pathways. (G) The subnetworks identified by PhenomeExpress. The DE genes of AS were enriched in response to the chemokine subnetwork and those of SZ were enriched in the regulation of the transcription subnetwork. (H) The top-ranked DE gene of AS or SZ between patients with OA and non-OA controls, BAG1, was validated by IHC staining. (I) The top-ranked DE gene of AS or SZ between patients with OA and non-OA controls, CD9, was validated by IHC staining. The quantification of positive cells from different zones (ie, SZ, MZ and DZ) is displayed by bar plots (replicates n=6), where the AS and SZ were hardly distinguished during IHC staining, therefore, the AS and SZ in Geo-seq results were merged together to only present as SZ in IHC staining. The scale bar: left, 500 μm; right, 50 μm. AS, articular surface; DZ, deep zone; GO, Gene Ontology; IHC, immunohistochemistry; InfC, inflammatory chondrocyte; MZ, middle zone; NS, no significant; OA, osteoarthritis; preInfC, pre-inflammatory chondrocyte; preFC, prefibrocartilage chondrocyte; preHTC, prehypertrophic chondrocyte; SZ, superficial zone; UMAP, uniform manifold approximation and projection. *p<0.05, **p<0.01, ***p<0.001.

    Article Snippet: After rinsing, sections were sealed with goat serum (ZSGB-BIO, China) for 20 min at room temperature and incubated with rabbit antihuman CD74 (1:50, ab181470, Abcam, UK), mouse antihuman BMP2 (1:100, 66383-1-Ig, Proteintech, China), rabbit antihuman IBSP (1:2000, ab270605, Abcam), rabbit antihuman CHI3L1 (1:200, ab255297, Abcam), rabbit antihuman GPR183 (1:200, ab150625, Abcam), rabbit antihuman BAG1 (1:100, 19064-1-AP, Proteintech), rrabbit antihuman CD9 (1:2000, 20597-1-AP, Proteintech) overnight at 4°C.

    Techniques: Immunohistochemistry